ribosomal operon sequencing
Studies of bacterial microbiota using short read sequencing (V1–V6 variable regions of the 16S rRNA gene) report identifications at the genus through phylum level. Long read sequencing (Oxford Nanopore MinION, PacBio SMRT) of near full-length ribosomal operons provides robust, strain-level identification for complex natural samples.
Below is a link to a database of bacterial ribosomal operons spanning the small subunit (16S) to the large subunit (23S) and everything between including the internal transcribed spacer region and associated tRNAs (such as tRNA-Ile, tRNA-Ala). This database was extracted from prokaryotic genome sequences in RefSeq at NCBI. The result is over 300,000 rRNA operons for your strain-level tastes!
The rRNA Operon Database was created by a collaboration between the following associations:
Department of Marine & Coastal Sciences, Rutgers–the State University of New Jersey in New Brunswick, NJ
U.S. Army Combat Capabilities Development Command - Chemical Biological Center, Aberdeen Proving Ground, MD
Department of Biochemistry & Microbiology, Rutgers–the State University of New Jersey in New Brunswick, NJ
Australian Institute of Marine Sciences, Queensland, Australia
U.S. Deptartment of Agriculture-Agricultural Research Services, Genetic Improvement for Fruits & Vegetables Laboratory, Chatsworth, NJ
The Joint Program Executive Office for Chemical Biological Nuclear & Radiological Defense, Aberdeen Proving Ground, MD