top of page
Search

Princeton: Iron Limitation and Membrane Remodeling in Roseobacter

Writer's picture: Ray SullivanRay Sullivan

Updated: Feb 2





Mo Seyedsayamdost (Mo LAB) in Princeton’s Dept. of Chemistry continues to develop the HiTES approach on agar (instead of liquid media) to identify the molecules and their roles in initiating interactions between marine macroalgae and the prevalent Roseobacter group species that closely associates with them, Roseovarius tolerans.  They generated a library of 32 potential marine algal metabolites and applied their Eco-HiTES approach to R. tolerans.  They then screened the metabolite library using UPLC-qTOF-MS and MetEX analysis and purified and structurally characterized the target metabolites using NMR and mass spectrometry.  They also performed RNA-seq to analyze transcriptional changes induced by myricetin treatment.  Their results are summarized as follows:


- R. tolerans responds to the algal metabolite myricetin by producing novel ceramide lipids called roseoceramides, triggering a switch from a planktonic, high-energy lifestyle to a sessile (lowered proton motive force), symbiotic lifestyle with the algal host.

- The metabolic shift towards roseoceramide production is driven by iron limitation, representing an unprecedented iron-starvation stress adaptation.


The flavonoid signaling observed in the algal-bacterial interaction may be ancestral to the rhizosphere interactions between land plants and rhizobacteria, where plant flavonoids initiate symbiosis.


Ganley JG, Seyedsayamdost MR. Iron limitation triggers roseoceramide biosynthesis and membrane remodeling in marine roseobacter. Proc Natl Acad Sci U S A. 2025 Jan 28;122(4):e2414434122. doi: 10.1073/pnas.2414434122. Epub 2025 Jan 23. PMID: 39847340.  https://www.pnas.org/doi/10.1073/pnas.2414434122

1 view0 comments

Comments

Rated 0 out of 5 stars.
No ratings yet

Add a rating

©2019 by Theobold Smith Society. Proudly created with Wix.com

bottom of page